Single cell bacterial staining-haibo bio
1. Smear: (1) Drop a drop of normal saline on a clean glass slide without grease (if it is a liquid bacterial suspension, it may not be dropped). (2) Pick up a few colonies with sterile sterilization inoculation loop into the physiological saline of the glass slide, and gently coat them into a thin bacterial membrane. If it is a bacterial solution specimen, it is directly coated with a sterile inoculation needle to form a bacterial membrane. 2. Drying: The coated specimens are generally naturally dried at room temperature.
If you need to accelerate drying, you can use the hot air above the flame of the alcohol lamp to heat it, avoid fire and excessive temperature.
3. Fixation: The flame heating method is commonly used for bacterial fixation
Pass the dry smear quickly through the flame 2 to 3 times, the temperature should not be too high, as the back of the slide glass touches the skin with heat and not hot.
4. Staining: The purpose of staining is to increase the contrast between the specimen and the environment, which is convenient for observation under a microscope.
Taking the staining of ordinary smear specimens as an example, it is necessary to add the required staining droplets on the specimen to cover the coating film to achieve the required staining time. Pour the staining solution, wash with water, and blot dry.
5. Mordant: Some dyeing needs to add mordant. In order to increase the affinity of the dye and the substance of the tested specimen, or to make the dye better fixed in the specimen.
The mordant can be used to fix the bacterial specimen, and can also be included in the fixative or staining solution. The iodine solution in Gram staining is the mordant.
6. Decolorization: Decolorization of colored dyes is called decolorization. Materials that can decolorize are called decolorizing agents.
In dyeing, the use of decolorizing agent may play a role as a solvent, and some may affect the ionization degree of bacterial proteins, change the nature and quantity of charge, and thus affect the dyeing quality of the dye.
The use of decolorizing agent is mainly to check the stability of the combination of dye and bacteria, as the identification of dyeing. If ethanol is used for Gram staining, it is to identify different bacteria.
When using a decolorizing agent, it is to drop the decolorizing agent on the stained specimen. After a certain period of time, the decolorizing agent is poured out and washed with water. Some bleaching must have a good time, otherwise it will not achieve the ideal purpose.
7. Counterstaining: In the case of differential staining, specimens that have been decolorized often need to be re-stained with the counterstain once, so that the counterstain has a sharp contrast with the initial color. Counter-staining should not be too strong, so as not to cover the initial color.
Valve Bag,Outer Valve Bag,Bulk Bag Valve,Block Bottom Outer Valve Bag
ZHEJIANG IMERIC ENVIRONMENTAL PROTECTION SCIENCE & TECHNOLOGY CO., LTD. , https://www.imeric-valvebags.com