Rabbit cartilage oligomeric matrix protein (COMP) ELISA test kit instruction manual

Rabbit cartilage oligomeric matrix protein (COMP) quantitative detection kit (ELISA) instruction manual [kit name] rabbit cartilage oligomeric matrix protein (COMP) quantitative detection kit (ELISA) [kit use] quantitative detection of rabbit serum, plasma And the content of cartilage oligomeric matrix protein (COMP) in related liquid samples. [Detection principle] The kit uses a double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). The standard, the test sample and the enzyme standard working solution are added to a transparent enzyme-labeled plate pre-coated with rabbit cartilage oligomeric matrix protein (COMP) antibody, and after incubation for a sufficient time, the unbound component is washed and removed. The developer A and B are sequentially added, and the color developing agent (TMB) is converted into a blue product under the catalysis of horseradish peroxidase (HRP), which turns yellow under the action of acid, and the color depth and the rabbit in the sample. The cartilage oligomeric matrix protein (COMP) concentration was positively correlated, and the OD value was measured at a wavelength of 450 nm. The rabbit cartilage oligomeric matrix protein (COMP) content in the sample was calculated based on the OD value of the standard and the sample. [Kit kit composition] 1 Enzyme label coating plate 12 holes × 8 strips 7 Developer A liquid 6mL2 Standard: 8μg / L 0.6mL 8 Developer B liquid 6mL3 20 times concentrated washing liquid 25mL 9 Stop liquid 6mL4 Standard Diluent 6mL 10 Instructions 1 part 5 Sample dilution 6mL 11 Sealing membrane 2 sheets 6 Enzyme standard reagent 10mL 12 Sealed bag 1 Remarks: Standards are diluted with standard dilutions to: 8, 4, 2, 1, 0.5 0.25 μg/L [required and not provided reagents and equipment] 1, 37 ° C incubator 2, standard size microplate reader 3, precision pipette and disposable tip 4, distilled water 5, disposable test tube 6, water absorption Paper [Operation Procedure] 1. Preparation: Remove the kit from the refrigerator and re-balance at room temperature for 30 minutes. 2. Dosing: Dilute the 20 times concentrated washing solution into the original washing solution with distilled water. 3. Add standard and sample to be tested: Take a sufficient number of enzyme label coated plates, fix them on the frame, set standard hole, sample hole and blank control hole respectively, record the position of each hole, in the standard hole Add 50 μL of the standard sample; add 10 μL of the sample to be tested first, and then add 40 μL of the sample diluent (ie, the sample is diluted 5 times); add 100 μL of the enzyme standard working solution to each well; blank control wells are not added. 4. Incubation: Incubate for 60 min in a 37 ° C water bath or incubator. 5. Wash the board: discard the liquid, pat dry on the absorbent paper, fill each well with the washing liquid, let stand for 1 min, remove the washing liquid, pat dry on the absorbent paper, and repeat the washing 5 times (you can also use the washing machine to press Instructions for washing the board). 6. Color development: 50 μL of the developer A solution was added to each well, and then 50 μL of the developer B solution was added, and the color was developed at 37 ° C for 15 minutes. 7. Termination: The enzyme plate was taken out, and 50 μL of the stop solution was added to each well to terminate the reaction (the color was changed from blue to yellow). 8. Measurement: Zeroing was performed with a blank hole, and the absorbance (OD value) of each well was measured with a wavelength of 450 nm within 15 minutes after termination. 9. Calculation: Calculate the linear regression equation of the standard curve according to the concentration of the standard product and the corresponding OD value, and then calculate the corresponding sample concentration on the regression equation according to the OD value of the sample. You can also use various application software to Calculation. The final concentration is the actual measured concentration multiplied by the dilution factor. [Sample Requirements] 1. The sample should not contain sodium azide (NaN3) because sodium azide (NaN3) is an inhibitor of horseradish peroxidase (HRP). 2. The specimens should be extracted as soon as possible after the collection, and the extraction should be carried out according to the relevant literature. The experiment should be carried out as soon as possible after extraction. If the test cannot be performed immediately, the specimen can be stored at -20 °C, but repeated freezing and thawing should be avoided. 3, the sample should be fully centrifuged, no hemolysis and particles. [Notes] 1. The experiment is carried out in strict accordance with the operation of the manual. The results of the experiment must be determined by the reading of the microplate reader. 2. If the enzyme label is not used up after being opened, it should be immediately placed in a sealed bag and desiccant. 3. It is recommended that all standards, samples and blanks be double-tested and averaged to reduce experimental error. 4. If the color is too light, the substrate incubation time can be extended appropriately. 5. In order to avoid cross-contamination, the standard product, sample and blank control should be replaced once for each additional one; the common components such as enzyme standard working solution, sample diluent and substrate should be cantilevered and should not touch micropores. ; Do not reuse the sealing film. 6. The kit is used during the shelf life. The reagents of different batches must not be mixed. 7. Substrate B is sensitive to light and avoids prolonged exposure to light. [Summary of Operation Procedure] Prepare reagents, samples and standards into the prepared sample, standard and enzyme standard working solution, wash the plate 5 times at 37 ° C for 60 minutes, add coloring solution A, B, and develop color at 37 ° C for 15 minutes. The reading OD value is calculated within 15 minutes after adding the stop solution. [Detection range] 0.25-8 μg/L [Specification] 96 persons/box [Storage] 2-8 °C, protected from light and moisture. [Validity Period] 6 months

Tumbler Holders

As the necessary item for bathroom, our tumbler holders are both attractive and practical. Lightweight and compact of the compartment provide enough space and organize your bathroom items well.Protective design to hold your tumbler, anti-dust, avoid bacteria breeding, keeps your toothpaste and brush dry, hygienic and healthy.

Tumbler Holders,Hanging Toothbrush Holder,Modern Toothbrush Holder,Single Toothbrush Holder

Kaiping Yufa Sanitary Ware Co.,ltd , https://www.yufafaucet.com