Cholesterol (CH) Elisa kit description parameters
2025-08-26 06:04:18
This Cholesterol (CH) ELISA Kit is designed for the quantitative determination of cholesterol levels in rat serum, plasma, cell culture supernatants, and other related liquid samples. The method employed is based on the double-antibody sandwich principle, ensuring high specificity and sensitivity. The microtiter plate is pre-coated with a purified monoclonal antibody specific to rat cholesterol. During the assay, the sample is added to the wells, allowing any cholesterol present to bind to the immobilized antibody. Subsequently, a horseradish peroxidase (HRP)-labeled secondary antibody is introduced, forming a complex of antibody-antigen-enzyme-labeled antibody. After thorough washing to remove unbound components, the substrate TMB is added. Under the catalytic action of HRP, TMB turns blue, and upon addition of an acidic stop solution, it changes to yellow. The intensity of the color is directly proportional to the concentration of cholesterol in the sample. The optical density (OD) is measured at 450 nm using a microplate reader, and the cholesterol concentration is determined by comparing the OD values to a standard curve generated from known concentrations. This kit provides a reliable and efficient way to assess cholesterol levels in various biological matrices, making it a valuable tool in research and diagnostic applications.
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