ELISA Protocol
ELISA is the abbreviation for Enzyme-Linked Immunosorbnent Assay. It is an immunoenzyme technology developed after immunofluorescence and radioimmunoassay. This technology has developed very rapidly since its introduction in the early 1970s, and it has been widely used in many fields of biology and medical sciences.
ELISA is based on immunological reaction, a highly sensitive test technique that combines the specific reactions of antigen and antibody with the efficient catalytic effect of enzyme on substrate. Since the reaction of antigen and antibody is carried out in the well of a solid phase carrier-polystyrene microtiter plate, after each reagent is added to the incubation, excess free reactants can be removed by washing to ensure the specificity of the test results With stability. In practical applications, through different designs, there are many specific method steps. That is: the indirect method for detecting antibodies (Figure a), the double antibody sandwich method for detecting antigen (Figure b), and the antigen competition method for detecting small molecule antigens or haptens, etc. More commonly used are ELISA double antibody sandwich method and ELISA indirect method.
Earloop Face Mask,Disposable Earloop Face Mask ,Surgical Masks Disposable,Civil Face Mask 3 Ply
ShenZhen Bri-Cloud Industrial Co.,Ltd , https://www.sobeautyhealth.com