(1) Take the morning glory, the seed coat is cracked after soaking in water, and the hand twist has obvious sticky and slippery feeling.
(2) Raw morning glory powder is yellowish brown. The seed coat epidermal cells are dark brown, irregular in shape, and the wall is microwave-like. Non-glandular single cells, yellow-brown, slightly curved, 50-240 μm long. The cotyledon fragments have secretory cavities, round or elliptical, 35-106 μm in diameter. Calcium oxalate cluster crystals are 10-25 μm in diameter. Palisade tissue fragments and brilliance Visible with time.
(3) Take 2 g of the morning glory control medicinal material, place it in a Soxhlet extractor, and extract with petroleum ether (60-90 ° C) for 2 hours under reflux. Discard the petroleum ether solution, evaporate the drug residue, and add chloroform-methanol ( 3: 1) The mixed solution is extracted for 6 hours. The extract recovers at least the amount of solvent, transfers it to a 10ml measuring bottle, wash the container with the same solvent to the same measuring bottle, and dilute to the mark, shake well to obtain the control medicinal solution. Then take the caffeic acid reference substance and the caffeic acid ethyl reference substance and add methanol to make a solution containing 1 mg per 1 ml as the reference substance solution. According to the TLC test, draw 10-20 μl of the test product solution and the control medicinal solution under the item (content determination), and 10 μl of the control solution, respectively, and spot them on the same high-efficiency silica gel G thin-layer plate. Methanol formic acid (100: 9: 4) was used as a developing agent, developed, taken out, and dried, sprayed with phosphomolybdic acid test solution, and heated at 110 ° C until the spots showed clear color. In the chromatogram of the test product, the same blue and black spots were displayed on the corresponding positions of the chromatogram of the control medicinal materials and the control substance.
Shanghai Ruiqi Biological Technology Co., Ltd. specializes in providing various reference products, with a complete range of products and competitive prices. 
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