Experimental principle of human macrophage inflammatory protein 1Î² (MIP-1Î² / CCL4) ELISA kit

2020-06-13 12:12:02

1. The level of human macrophage inflammatory protein 1Î² (MIP-1Î² / CCL4) ELISAKit was determined by double antibody sandwich method.

2. First coat the microplate with purified human macrophage inflammatory protein 1Î² (MIP-1Î² / CCL4) ELISAKit antibody to make a solid phase antibody.

3. Then add macrophage inflammatory protein 1Î² (MIP-1Î² / CCL4) ELISAKit to the microwells coated with mAb, and then add HRP-labeled macrophage inflammatory protein 1Î² (MIP-1Î² / CCL4) ELISAKit antibody The combination forms an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid.

4. The color depth is positively correlated with the macrophage inflammatory protein 1Î² (MIP-1Î² / CCL4) ELISAKit in the sample.

5. Finally, the absorbance (OD value) is measured with a microplate reader at a wavelength of 450 nm, and the concentration of human macrophage inflammatory protein 1Î² (MIP-1Î² / CCL4) ELISAKit in the sample is calculated by a standard curve

Objective: To determine the content of macrophage inflammatory protein 1Î² (MIP-1Î² / CCL4) ELISAKit in human serum, plasma and related fluid samples.

6. Serum: Blood coagulates naturally at room temperature for 10-20 minutes, centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully and centrifuge again if a precipitate appears during storage. Avoid any cell stimulation during the operation. Use test tubes free of pyrogens and endotoxins.

7. Plasma: EDTA or sodium citrate should be selected as the anticoagulant according to the requirements of the specimen. After mixing for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm) to remove particles. Collect the supernatant carefully, and centrifuge again if there is any precipitate.

safety:
1. Avoid direct contact with stop solution and substrate. If you accidentally come into contact with these liquids, please rinse them with water as soon as possible.
2. Do not eat, drink, smoke or use cosmetics during the experiment.
3. Do not touch any ingredients in the kit with your mouth.

4 Keep Elisa kit away from children

Bring your own items:

1. 37 â„ƒ incubator

2. Standard specification microplate reader

3. Precision pipette and disposable tip

4. Distilled water

5. Disposable test tube

6. Absorbent paper

Storage conditions and validity period:

1. Kit storage :; 2-8 â„ƒ.

2. Validity: 6 months