Method for measuring growth rate of solid medium
2025-07-13 01:08:53
The main focus of today's procedure is the method for measuring the growth rate of microorganisms on solid media using a qualitative approach.
First, the control strain was inoculated into a non-selective broth and allowed to grow overnight. For the test culture medium, a 1 µL loopful of the bacterial suspension was used to streak a parallel line on the surface of the agar. For molds, a sterilized inoculation needle was used to directly pick up the culture and inoculate it onto the test medium. Once inoculated, all plates were incubated under the conditions specified by the standard, such as temperature, humidity, and time.
After incubation, the growth was visually assessed and scored as follows:
- 0: No visible growth
- 1: Very weak or minimal growth
- 2: Good and clear growth
This scoring system helps determine whether the test medium supports the growth of the microorganism effectively compared to the control.
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