Human granulocyte colony-stimulating factor (G-CSF) enzyme-linked immunoassay kit instruction manual

Detection range: 96T20ng/L - 600ng/L Use purpose: This kit is used to determine the content of granulocyte colony-stimulating factor (G-CSF) in human serum, plasma and related liquid samples. Experimental principle This kit uses the double antibody sandwich method to determine the level of human granulocyte colony-stimulating factor (G-CSF) in the specimen. The microporous plate was coated with purified human granulocyte colony-stimulating factor (G-CSF) antibody to prepare a solid phase antibody, and granulocyte colony-stimulating factor (G-CSF) was sequentially added to the microcapsule of the coated monoclonal antibody, and then The HRP-labeled granulocyte colony-stimulating factor (G-CSF) antibody binds to form an antibody-antigen-enzyme-labeled antibody complex, which is thoroughly washed and then added to the substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The color depth is positively correlated with the granulocyte colony stimulating factor (G-CSF) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of human granulocyte colony stimulating factor (G-CSF) in the sample was calculated from a standard curve. Kit composition 1 30 times concentrated washing solution 20ml × 1 bottle 7 Stop solution 6ml × 1 bottle 2 enzyme standard reagent 6ml × 1 bottle 8 standard product (1200ng / L) 0.5ml × 1 bottle 3 enzyme label coating plate 12 holes × 8 strips 9 standard dilutions 1.5ml × 1 bottle 4 sample dilution 6ml × 1 bottle 10 instructions 1 part 5 developer A liquid 6ml × 1 bottle 11 sealing film 2 sheets 6 coloring agent B liquid 6ml × 1 Bottle 12 sealed bag 1

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