Anaerobic environment formation in the operation room of Xinzhuang anaerobic incubator
The YQX-11 Anaerobic Incubator from Xinzhuang (LNB) brand, manufactured by Shanghai Xinzhuang Instrument Co., Ltd., is a specialized device designed for cultivating and handling bacteria in an oxygen-free environment. It allows the growth of even the most challenging anaerobic organisms, significantly reducing the risk of exposure to oxygen during operations.
When setting up the anaerobic incubator, it's important to place it in a stable location with minimal temperature fluctuations. Avoid direct sunlight and keep it away from heat sources to ensure optimal performance. The unit should be placed on a level surface for stability.
Gas cylinders and nitrogen bottles should be secured in a safe and steady position. Install the pressure regulators (including pressure gauges) properly and make sure they are positioned correctly for easy access and monitoring.
Connect the gas lines carefully, and check for any potential leaks. If necessary, use sealant at each connection point to ensure airtight sealing and prevent contamination or loss of gas pressure.
Creating an anaerobic environment inside the incubator involves several key steps:
- Place all required tools and accessories in the operating chamber. Insert two non-toxic plastic bags into the chamber for gas exchange purposes.
- Turn on the power supply and activate the temperature controller. Set the desired temperature and safety limits accordingly.
- Place 1000g of sealed palladium pellets and 500g of desiccant in the chamber, along with a sealed Meilan indicator for oxygen detection.
- Close both the internal and external doors of the sampling chamber and perform a vacuum check to ensure airtight conditions.
- Perform the first nitrogen gas exchange: insert a rubber tube into the air inlet and connect it to one of the plastic bags. Open the nitrogen valve, fill the bag with nitrogen, close the valve, and seal the bag.
- Secure the latex gloves on the observation plate flange and tighten them properly.
- Transfer the nitrogen-filled plastic bag into the operating chamber and release the gas completely.
- Repeat the nitrogen exchange process for the second time. During this step, the sampling chamber should be evacuated, and the exhaust valve should be operated using the foot switch as needed.
- For the third gas exchange, use a mixed gas consisting of 85% nitrogen, 10% hydrogen, and 5% carbon dioxide. Adjust the air path to allow the mixed gas to flow into the plastic bag. Once filled, regulate the flow rate through the flow meter to approximately 10 ml per minute and gradually release the gas into the chamber.
- After three rounds of gas exchange, the oxygen level in the chamber should be extremely low. At this point, open the deoxidizing agent and turn on the catalytic deoxygenation system. Monitor the Meilan indicator for color change after one hour to confirm successful anaerobic conditions.
- Finally, activate the UV sterilization lamp to disinfect the interior. The duration of sterilization can vary depending on the specific experimental requirements.
Once the anaerobic environment is established, you can proceed with the inoculation and cultivation of microbial strains:
- Ensure the sampling chamber door is closed securely.
- Open the outer sampling door, place the culture sample inside, and then close the outer door immediately.
- Evacuate the sampling chamber using a vacuum pump until the pressure reaches at least 500 mmHg (66 kPa). Repeat this process three times to ensure a stable anaerobic atmosphere.
If you're interested in learning more about solid-phase extraction technology or other laboratory equipment, Shanghai Xinzhuang Instrument Co., Ltd. offers expert guidance. Their LNB brand products are known for their high stability, reliability, and quality. With a strong commitment to customer satisfaction, they continue to build trust through superior performance and service. Contact us today to discover how we can support your research and laboratory needs. Welcome to Shanghai Xinzhuang Instrument – where quality meets innovation.
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