Shanghai Jiapeng analytical gel imaging system steps

The steps of the gel imaging system:

1. Turn on the gel imaging system switch.

2. Turn on the computer, turn it on and enter the imaging software.

ECL shooting

Switch the shooting mode to “ECL mode” and turn the filter wheel to the ECL position. Choose the appropriate shooting resolution (both machines with pixel merging function have this feature), click "Start". Place the sample in the middle of the sample stage, adjust the focal length of the lens so that the sample occupies about 80% of the window, then click "Auto Exposure", tick off the "negative film" and adjust the focus to make the sample image in the preview window clear. (The aperture Large, the shorter the time required for automatic exposure.) And take a single frame to shoot a photo of Mark. After the reflected white light is turned off, the nitrocellulose membrane placed on the chemiluminescent image forming plate is uniformly added with the luminescent liquid. Set the shooting mode to “Regular Integration”, check “Negative”, set the time and number of sheets, and click the “Shooting” button.

Ordinary gel shooting

1. Switch the shooting mode to “Normal Mode” and turn the filter wheel to the UV position (no adjustment is required without the green lens wheel).

2. Select the appropriate shooting resolution (the machine has a pixel merging function), click "Start".

3. DNA gel shooting: Place the DNA glue in the middle of the UV station, adjust the focal length so that the sample occupies about 80% of the window, then click “Auto Exposure” and adjust the focus to make the sample image in the preview window clear. Then turn off the reflection. White light, turn on the transmission of UV, and fine-tune to ensure clear in the UV.

Protein gel shooting: Place the protein gel in the middle of the unfolded white plate, turn off the reflected white light, turn on the transmitted white light, then click “Auto Exposure” and adjust the focus to make the sample image in the preview window clear.

4. Click the “shoot” button in the software interface.